Horseradish and soybean peroxidases: comparable tools for alternative niches?

1 Horseradish and Soybean Peroxidases : Comparable Tools for Alternative 1 Niches ?

Peroxidase Activity of a c‐Type Cytochrome b 5 in the Non‐Native State is Comparable to that of Native Peroxidases

The design of artificial metalloenzymes has achieved tremendous progress, although few designs can achieve catalytic performances comparable to that of native enzymes. Moreover, the structure and function of artificial metalloenzymes in non-native states has rarely been explored. Herein, we found that a c-type cytochrome b5 (Cyt b5), N57C/S71C Cyt b5, with heme covalently attached to the protei...

Direct voltammetry and catalysis with Mycobacterium tuberculosis catalase-peroxidase, peroxidases, and catalase in lipid films.

Stable films of dimyristoylphosphatidylcholine and M. tuberculosis catalase-peroxidase (KatG), several peroxidases, myoglobin, and catalase showed reversible FeIII/FeII voltammetry on pyrolytic graphite electrodes and catalytic current for hydrogen peroxide and oxygen. Amperometric responses for these films to H2O2 at 0 V are likely to contain significant contributions from catalytic reduction ...

Hydroxyl-radical production in physiological reactions. A novel function of peroxidase.

Peroxidases catalyze the dehydrogenation by hydrogen peroxide (H2O2) of various phenolic and endiolic substrates in a peroxidatic reaction cycle. In addition, these enzymes exhibit an oxidase activity mediating the reduction of O2 to superoxide (O2.-) and H2O2 by substrates such as NADH or dihydroxyfumarate. Here we show that horseradish peroxidase can also catalyze a third type of reaction tha...

Elimination of 4-chlorophenol from Water Solutions Using Commercial Peroxidases

A comparative study of the two most widely used commercial peroxidases (E.C. 1.11.1.7) for removing 4-chlorophenol from aqueous industrial effluents is presented. Both the peroxidases tested, horseradish peroxidase (HRP) and soybean peroxidase (SBP), showed maximal removal efficiency in a neutral pH medium although they maintained more than 70 % of their activity in a pH range of between 6.0 an...